Difference between revisions of "Part:BBa P1011:Design"
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===Design Notes===
 
===Design Notes===
  
−
This is identical to BBa_P1010 except that ccdA- has been deleted since its presence is not necessary for ccdB's function as a toxin.  Some sequence from the 5' end of BBa_P1010 has also been deleted in order to eliminate a BamHI site.
+
This part is derived from BBa_P1010.  
  
 +
The following changes have been made.
 +
#''ccdA-'' has been deleted since its presence is not necessary for ccdB's function as a toxin. 
 +
#Some sequence from the 5' end of BBa_P1010 has also been deleted in order to eliminate a BamHI site.
 +
#Silent mutations have been introduced in order to eliminate useful restriction sites and rare codons.
  
 
===Source===
 
===Source===

Revision as of 23:09, 17 August 2006

ccdB cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is derived from BBa_P1010.

The following changes have been made.

  1. ccdA- has been deleted since its presence is not necessary for ccdB's function as a toxin.
  2. Some sequence from the 5' end of BBa_P1010 has also been deleted in order to eliminate a BamHI site.
  3. Silent mutations have been introduced in order to eliminate useful restriction sites and rare codons.

Source

BBa_P1010

References