Difference between revisions of "Part:BBa K1033928"
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<partinfo>BBa_K1033928 short</partinfo> | <partinfo>BBa_K1033928 short</partinfo> | ||
| − | aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue has significant sequence homologies with proteins in the GFP family. | + | aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone ''Actinia equina''. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue has significant sequence homologies with proteins in the GFP family. |
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| + | ===Source=== | ||
| + | The protein was first extracted and characterized by Shkrob et. al. 2005 under the name aeCP597. This version is codon optimized for '' E coli'' by Bioneer Corp. | ||
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| + | Below to the left is the expressed aeBlue in ''E coli''. To the right comparison of aeBlue with other chromoproteins available from the registry. | ||
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| + | [[Image:Aeblue_plate_small.jpg.png|360px]] | ||
| + | [[Image:UUChromo.jpg|500px]] | ||
| + | <br>'''iGEM12_Uppsala_University:''' ''Left picture:'' Visual color expression of aeBlue(<partinfo>BBa_K864401</partinfo>). | ||
| + | ''Right picture:'' The Uppsala chromoprotein collection and RFP. Pellets of bacteria expressing chromoproteins eforRed (<partinfo>BBa_K592012</partinfo>), RFP (<partinfo>BBa_E1010</partinfo>), cjBlue (<partinfo>BBa_K592011</partinfo>), aeBlue (<partinfo>BBa_K864401</partinfo>), amilGFP (<partinfo>BBa_K592010</partinfo>) and amilCP (<partinfo>BBa_K592009</partinfo>). | ||
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| + | ===References=== | ||
| + | [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316306/]Shkrob, M.A., Yanushevich, Y.G., Chudakov, D.M., Gurskaya, N.G., Labas, Y.A., Poponov, S.Y., Mudrik, N.N., Lukyanov, S., Lukyanov, K.A., 2005. Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem. J. 392, 649–654. | ||
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Latest revision as of 15:57, 31 October 2013
aeBlue, blue chromoprotein (incl RBS, J23110)
aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue has significant sequence homologies with proteins in the GFP family.
Source
The protein was first extracted and characterized by Shkrob et. al. 2005 under the name aeCP597. This version is codon optimized for E coli by Bioneer Corp.
Below to the left is the expressed aeBlue in E coli. To the right comparison of aeBlue with other chromoproteins available from the registry.
iGEM12_Uppsala_University: Left picture: Visual color expression of aeBlue(BBa_K864401).
Right picture: The Uppsala chromoprotein collection and RFP. Pellets of bacteria expressing chromoproteins eforRed (BBa_K592012), RFP (BBa_E1010), cjBlue (BBa_K592011), aeBlue (BBa_K864401), amilGFP (BBa_K592010) and amilCP (BBa_K592009).
References
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316306/]Shkrob, M.A., Yanushevich, Y.G., Chudakov, D.M., Gurskaya, N.G., Labas, Y.A., Poponov, S.Y., Mudrik, N.N., Lukyanov, S., Lukyanov, K.A., 2005. Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem. J. 392, 649–654.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]