Difference between revisions of "Part:BBa K1088006"
Line 4: Line 4:
 
This composite part consists of 5 sub parts
 
This composite part consists of 5 sub parts
  
−
A strong constitutive promoter: [https://parts.igem.org/Part:BBa_J23100 BBa_J23100]
+
A strong constitutive promoter: [https://parts.igem.org/Part:BBa_J23100 BBa_J23100]  
−
A strong RBS: [https://parts.igem.org/Part:BBa_J15001 BBa_J15001]
+
A strong RBS: [https://parts.igem.org/Part:BBa_J15001 BBa_J15001]  
−
The coding region of ''dxs'' from ''B. subtilis'': [https://parts.igem.org/Part:BBa_K1088000 BBa_K1088000]
+
The coding region of ''dxs'' from ''B. subtilis'': [https://parts.igem.org/Part:BBa_K1088000 BBa_K1088000]  
−
A 10 amino acid protein linker: [https://parts.igem.org/Part:BBa_K105012 BBa_K105012]
+
A 10 amino acid protein linker: [https://parts.igem.org/Part:BBa_K105012 BBa_K105012]  
−
The AmilCP chromoprotein: [https://parts.igem.org/Part:BBa_K592009 BBa_K592009]
+
The AmilCP chromoprotein: [https://parts.igem.org/Part:BBa_K592009 BBa_K592009]  
  
 
The idea was to detect expression of the Dxs protein by the reporter amilCP fused via a linker to the Dxs protein. Unfortunately it seems that the amilCP protein does not work well as a protein-fusion reporter, and the color change was vague.  
 
The idea was to detect expression of the Dxs protein by the reporter amilCP fused via a linker to the Dxs protein. Unfortunately it seems that the amilCP protein does not work well as a protein-fusion reporter, and the color change was vague.  

Revision as of 18:08, 28 October 2013

B. subtilis dxs-AmilCP fusion (constitutive promoter)

This composite part consists of 5 sub parts

A strong constitutive promoter: BBa_J23100 A strong RBS: BBa_J15001 The coding region of dxs from B. subtilis: BBa_K1088000 A 10 amino acid protein linker: BBa_K105012 The AmilCP chromoprotein: BBa_K592009

The idea was to detect expression of the Dxs protein by the reporter amilCP fused via a linker to the Dxs protein. Unfortunately it seems that the amilCP protein does not work well as a protein-fusion reporter, and the color change was vague.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1765
    Illegal PstI site found at 1166
    Illegal PstI site found at 1612
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1765
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1166
    Illegal PstI site found at 1612
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1765
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1765
    Illegal PstI site found at 1166
    Illegal PstI site found at 1612
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1765
    Illegal PstI site found at 1166
    Illegal PstI site found at 1612
    Illegal NgoMIV site found at 1065
    Illegal AgeI site found at 958
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 907
    Illegal SapI.rc site found at 1606