Difference between revisions of "Part:BBa K1139026:Design"

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===Source===
 
===Source===
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<i>BAD</i> promoter from (<partinfo>BBa_I13453</partinfo>), M13 genes and origin from <partinfo>BBa_K1139018</partinfo>, <i>GFP</i> from <partinfo>BBa_E0040</partinfo>, rbs from <partinfo>BBa_B0030</partinfo>, Double terminator from <partinfo>BBa_B0015</partinfo> are combined.
+
<i>BAD</i> promoter from <partinfo>BBa_I13453</partinfo>, M13 genes and origin from <partinfo>BBa_K1139018</partinfo>, <i>GFP</i> from <partinfo>BBa_E0040</partinfo>, rbs from <partinfo>BBa_B0030</partinfo>, Double terminator from <partinfo>BBa_B0015</partinfo> are combined.
  
 
===References===
 
===References===

Latest revision as of 17:23, 28 October 2013


Pbad-M13-Plac-GFP on pSB3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 238
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 7425


Design Notes

sequence confirmed

Source

BAD promoter from BBa_I13453, M13 genes and origin from BBa_K1139018, GFP from BBa_E0040, rbs from BBa_B0030, Double terminator from BBa_B0015 are combined.

References

1. Atsushi Higashitani. Nahoko Higashitani. Kensuke Horiuchi, DNA Replication in Filamentous Bacteriophage, Protein, Nucleic Acid and Enzyme(1994), vol39:2189-2197.
2. Kensuke Horiuchi, Origin of DNA replication of filamentous coliphages, Jpn. J. Genet(1990), vol65:225-241.
3. ME Ortiz. D Endy., Engineered cell-cell communication via DNA messaging, J. Biol. Engineering(2012), 6-16.
4. A Goñi-Moreno, M Amos, F de la Cruz, Multicellular Computing Using Conjugation for Wiring, PLOS ONE(2013), Vol8.:Issue6:e65986.
5. M Shintani. N Fukushima. M Tezuka. H Yamane. H Nojiri, Conjugative transfer of the IncP-7 carbazole degradative plasmid, pCAR1, in river water samples, Biotechnol Lett (2008). vol30:117-122
6.H Nojiri Structural and Molecular Genetic Analyses of the Bacterial Carbazole Degradation System Biosci, Biotechnol, Biochem, 76(1), 1-18, 2012