Difference between revisions of "Part:BBa K1104250"
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<partinfo>BBa_K1104250 short</partinfo> | <partinfo>BBa_K1104250 short</partinfo> | ||
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| + | <partinfo>BBa_K1104250 parameters</partinfo> | ||
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| + | ===Usage and Biology=== | ||
[[Image:NYMU_O12.png|frame|center|'''OxyR-included circuit(Device1+Device2)''']] | [[Image:NYMU_O12.png|frame|center|'''OxyR-included circuit(Device1+Device2)''']] | ||
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Revision as of 10:46, 27 October 2013
OxyR device
Functional Parameters
Usage and Biology
OxyR-included circuit(Device1+Device2)
In order to enhance the strength and sensitivity of ROS-sensing promoters, we make E. coli continuously produce inactivated OxyR, so that ROS can affect promoters more easily by adding a device: Device1.
Device1 in order to enhance the effect of ROS on E. coli is composed of :
- a constitutive promoter(Part:BBa_J23102)
- RBS(Part:BBa_B0034)
- activator OxyR(Part:BBa_K1104200)
- double terminator [https://parts.igem.org/Part:BBa_B0015 Part:BBa_B0015
 Device1 a constitutive promoter(Part:BBa_J23102)+ RBS(Part:BBa_B0034)+ activator OxyR(Part:BBa_K1104200)+ double terminator Part:BBa_B0015 |
 Device2 a sensor (OxyR-induced promoter, including TrxCp, HemHp, sufA, AhpCp1000, AhpCp2D1, AhpCp2, AhpCpD1, AhpCp1, DsbGp) + reporter (Part:BBa_E0840) |
Putting Device1 BBa_K1104250 upstream to promoter AhpCp2D1(Part:BBa_K1104204) remarkably enhanced the strength of promoter AhpCp2D1(Part:BBa_K1104204) as our experiment results shows.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]