Difference between revisions of "Part:BBa K1104204:Experience"
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===Device1 testing=== | ===Device1 testing=== | ||
| + | [[Image:NYMU_O12.png|frame|center|'''OxyR-included circuit(Device1+Device2)''']] | ||
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| + | In OxyR-included circuit, Device2 is composed of sensor (OxyR-induced promoter, including TrxCp, HemHp, sufA, AhpCp1000, AhpCp2D1, AhpCp2, AhpCpD1, AhpCp1, DsbGp) plus reporter ([https://parts.igem.org/Part:BBa_E0840 Part:BBa_E0840]). In this case we use AhpCp2D1. | ||
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|[[Image:NYMU_O1.png|frame|center|'''Device1 ([https://parts.igem.org/Part:BBa_K1104250 Part:BBa_K1104250])'''<br>a constitutive promoter([https://parts.igem.org/Part:BBa_J23102 Part:BBa_J23102])+ RBS([https://parts.igem.org/Part:BBa_B0034 Part:BBa_B0034])+ activator OxyR([https://parts.igem.org/Part:BBa_K1104200 Part:BBa_K1104200])+ double terminator [https://parts.igem.org/Part:BBa_B0015 Part:BBa_B0015]]] | |[[Image:NYMU_O1.png|frame|center|'''Device1 ([https://parts.igem.org/Part:BBa_K1104250 Part:BBa_K1104250])'''<br>a constitutive promoter([https://parts.igem.org/Part:BBa_J23102 Part:BBa_J23102])+ RBS([https://parts.igem.org/Part:BBa_B0034 Part:BBa_B0034])+ activator OxyR([https://parts.igem.org/Part:BBa_K1104200 Part:BBa_K1104200])+ double terminator [https://parts.igem.org/Part:BBa_B0015 Part:BBa_B0015]]] | ||
Revision as of 10:16, 27 October 2013
Results
Relative promoter strength testing
This is the relative promoter strength of BBa_K1104204 under different H202 concentrations tested using BBa_K1104244 (BBa_K1104204 +E0840) and found that there is a trend where the higher the H202 concentration, the higher the promoter expression:
Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101, and the AhpC2D1 promoter shows higher strength than the AhpCp 1000 which is an effective improvement :
Promoter testing of ahpC promoter (Part:K362001) and its improvements.
Device1 testing
In OxyR-included circuit, Device2 is composed of sensor (OxyR-induced promoter, including TrxCp, HemHp, sufA, AhpCp1000, AhpCp2D1, AhpCp2, AhpCpD1, AhpCp1, DsbGp) plus reporter (Part:BBa_E0840). In this case we use AhpCp2D1.
 Device1 (Part:BBa_K1104250) a constitutive promoter(Part:BBa_J23102)+ RBS(Part:BBa_B0034)+ activator OxyR(Part:BBa_K1104200)+ double terminator Part:BBa_B0015 |
 Device2 a sensor (OxyR-induced promoter, including TrxCp, HemHp, sufA, AhpCp1000, AhpCp2D1, AhpCp2, AhpCpD1, AhpCp1, DsbGp) + reporter (Part:BBa_E0840) |
Device1 in order to enhance the effect of ROS on E. coli is added ahead: a constitutive promoter(Part:BBa_J23102), RBS(Part:BBa_B0034), activator OxyR(Part:BBa_K1104200),and double terminator Part:BBa_B0015. Putting Device1 device:BBa_K1104250 upstream to promoter AhpCp2D1(Part:BBa_K1104204) remarkably enhanced the strength of promoter AhpCp2D1(Part:BBa_K1104204) as our experiment results shows:
