Difference between revisions of "Part:BBa K1104204:Experience"
Line 7: Line 7:
 
Promoter testing of ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) and its improvements.
 
Promoter testing of ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) and its improvements.
 
[[File: NYMU_AA.png|frame|center]]
 
[[File: NYMU_AA.png|frame|center]]
−
 
−
Device1 in order to enhance the effect of ROS on ''E. coli'' is added ahead: a constitutive promoter([https://parts.igem.org/Part:BBa_J23102 Part:BBa_J23102]), RBS([https://parts.igem.org/Part:BBa_B0034 Part:BBa_B0034]), activator OxyR([https://parts.igem.org/Part:BBa_K1104200 Part:BBa_K1104200]),and double terminator [https://parts.igem.org/Part:BBa_B0015 Part:BBa_B0015]. Putting Device1 [https://parts.igem.org/Part:BBa_K1104250 device:BBa_K1104250] upstream to promoter AhpCp2D1([https://parts.igem.org/Part:BBa_K1104204 Part:BBa_K1104204]) remarkably enhanced the strength of promoter AhpCp2D1([https://parts.igem.org/Part:BBa_K1104204 Part:BBa_K1104204]) as our experiment results shows:
 
−
[[Image:NYMU_2D1O.png|frame|center]]
 
  
 
{|
 
{|
Line 15: Line 12:
 
|[[Image:NYMU_O2.png|frame|center|'''Device2'''<br>a sensor (OxyR-induced promoter, including TrxCp, HemHp, sufA, AhpCp1000, AhpCp2D1, AhpCp2, AhpCpD1, AhpCp1, DsbGp) + reporter ([https://parts.igem.org/Part:BBa_E0840 Part:BBa_E0840])]]
 
|[[Image:NYMU_O2.png|frame|center|'''Device2'''<br>a sensor (OxyR-induced promoter, including TrxCp, HemHp, sufA, AhpCp1000, AhpCp2D1, AhpCp2, AhpCpD1, AhpCp1, DsbGp) + reporter ([https://parts.igem.org/Part:BBa_E0840 Part:BBa_E0840])]]
 
|}
 
|}
 +
 +
Device1 in order to enhance the effect of ROS on ''E. coli'' is added ahead: a constitutive promoter([https://parts.igem.org/Part:BBa_J23102 Part:BBa_J23102]), RBS([https://parts.igem.org/Part:BBa_B0034 Part:BBa_B0034]), activator OxyR([https://parts.igem.org/Part:BBa_K1104200 Part:BBa_K1104200]),and double terminator [https://parts.igem.org/Part:BBa_B0015 Part:BBa_B0015]. Putting Device1 [https://parts.igem.org/Part:BBa_K1104250 device:BBa_K1104250] upstream to promoter AhpCp2D1([https://parts.igem.org/Part:BBa_K1104204 Part:BBa_K1104204]) remarkably enhanced the strength of promoter AhpCp2D1([https://parts.igem.org/Part:BBa_K1104204 Part:BBa_K1104204]) as our experiment results shows:
 +
[[Image:NYMU_2D1O.png|frame|center]]

Revision as of 10:12, 27 October 2013

Results

This is the relative promoter strength of BBa_K1104204 under different H202 concentrations tested using BBa_K1104244 (BBa_K1104204 +E0840) and found that there is a trend where the higher the H202 concentration, the higher the promoter expression:

NYMU AhpCp2D1 strength.png

Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101, and the AhpC2D1 promoter shows higher strength than the AhpCp 1000 which is an effective improvement :

Comparison between ahpC and its improvements. (Part:BBa_K1104244)

Promoter testing of ahpC promoter (Part:K362001) and its improvements.

NYMU AA.png
Device1 (Part:BBa_K1104250)
a constitutive promoter(Part:BBa_J23102)+ RBS(Part:BBa_B0034)+ activator OxyR(Part:BBa_K1104200)+ double terminator Part:BBa_B0015
Device2
a sensor (OxyR-induced promoter, including TrxCp, HemHp, sufA, AhpCp1000, AhpCp2D1, AhpCp2, AhpCpD1, AhpCp1, DsbGp) + reporter (Part:BBa_E0840)

Device1 in order to enhance the effect of ROS on E. coli is added ahead: a constitutive promoter(Part:BBa_J23102), RBS(Part:BBa_B0034), activator OxyR(Part:BBa_K1104200),and double terminator Part:BBa_B0015. Putting Device1 device:BBa_K1104250 upstream to promoter AhpCp2D1(Part:BBa_K1104204) remarkably enhanced the strength of promoter AhpCp2D1(Part:BBa_K1104204) as our experiment results shows:

NYMU 2D1O.png