Difference between revisions of "Part:BBa K1104250"

Revision as of 09:34, 27 October 2013

OxyR device

OxyR-included circuit(Device1+Device2)

In order to enhance the strength and sensitivity of ROS-sensing promoters, we make E. coli continuously produce inactivated OxyR, so that ROS can affect promoters more easily by adding a device: Device1.

Device1 in order to enhance the effect of ROS on E. coli is composed of :

1. a constitutive promoter(Part:BBa_J23102)
2. RBS(Part:BBa_B0034)
3. activator OxyR(Part:BBa_K1104200)
4. double terminator [https://parts.igem.org/Part:BBa_B0015 Part:BBa_B0015

Putting Device1 BBa_K1104250 upstream to promoter AhpCp2D1(Part:BBa_K1104204) remarkably enhanced the strength of promoter AhpCp2D1(Part:BBa_K1104204) as our experiment results shows.

Device1 a sensor (OxyR-induced promoter, including TrxCp, HemHp, sufA, AhpCp1000, AhpCp2D1, AhpCp2, AhpCpD1, AhpCp1, DsbGp) + reporter (Part:BBa_E0840)

Device2 a constitutive promoter(Part:BBa_J23102)+ RBS(Part:BBa_B0034)+ activator OxyR(Part:BBa_K1104200)+ double terminator Part:BBa_B0015

Sequence and Features