Difference between revisions of "Part:BBa K1067007"
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<partinfo>BBa_K1067007 short</partinfo> | <partinfo>BBa_K1067007 short</partinfo> | ||
| − | Non leaky pBAD promoter with araC constitutively expressed. This biobrick is the same as [[part:BBa_K808000]] but with a change | + | Non leaky pBAD promoter with araC constitutively expressed. This biobrick is the same as [[part:BBa_K808000]] but with a change in pBAD promoter and another sequence annotation. The promoter is taken from the [[pBAD SPL]] col. 10 from [http://2013.igem.org/Team:DTU-Denmark 2013 DTU iGEM team]. |
===Characterization=== | ===Characterization=== | ||
Revision as of 12:48, 12 October 2013
Tight pBAD with araC
Non leaky pBAD promoter with araC constitutively expressed. This biobrick is the same as part:BBa_K808000 but with a change in pBAD promoter and another sequence annotation. The promoter is taken from the pBAD SPL col. 10 from [http://2013.igem.org/Team:DTU-Denmark 2013 DTU iGEM team].
Characterization
Non-induced activity vs inducted activity for a variety of promoters built from the pBAD_SPL. This biobrick (BBa_K1067007) is built with colony 10. The diagonal line represents 1:1, on which the constitutive reference promoter sits. The horizontal blue line represents the induced activity of BBa_K808000.
For further characterization and information see the pBAD SPL page or go to the 2013 DTU [http://2013.igem.org/Team:DTU-Denmark/pBAD_SPL wiki].
Usage and Biology
- Expression of lethal proteins
- Tight control
- Direct proteins to the periplasm, see Part:BBa_K1067009
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1182
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1182
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1182
Illegal AgeI site found at 979 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961