Difference between revisions of "Part:BBa K1172915"

 
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Biosafety-System M (TetR)
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Biosafety-System TetOR alive
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1172915 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1172915 SequenceAndFeatures</partinfo>
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[[File:Team-Bielefeld-Biosafeyt-System_TetORalive-TetOGFP-OD.jpg|600px|thumb|center|'''Figure 12:''' Characterization of the bacterial growth of the TetO promoter with GFP (<bbpart>BBa_E0040</bbpart>) in the Biosafety-Strain K-12 ∆alr ∆dadX. The M9 media was supplemented with 5 mM D-alanine. It can be seen, that the bacteria grow faster on M9 minimal media glucose than on M9 minimal media glycerol. ]]
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[[File:Team-Bielefeld-Biosafety-System-Tet2Flourescence.jpg|600px|thumb|center|'''Figure 13:''' Characterization of the fluorescence of TetO promoter with GFP (<bbpart>BBa_E0040</bbpart>) in the Biosafety-Strain K-12 ∆alr ∆dadX. The bacteria were cultivated on M9 minimal media with 5 mM D-alanine supplemented..]]
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[[File:Team-Bielefeld-Biosafeyt-System-TetORalive-specTetGFP.jpg|600px|thumb|center|'''Figure 14:''' Specific growth rate of GFP behind the TetO promoter by the use of different carbon sources.]]
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==='''Characterization of the Biosafety-System TetOR alive'''===
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[[File:Team-Bielefeld-Biosafety-System-TetORalive-ODALL.jpg|600px|thumb|center|'''Figure 15:''' Characterization of the bacterial growth of the Biosafety-System on M9 minimal media glycerol. The figure compares the wild tpye K-12 and the Biosafety-Strain K-12 ∆alr ∆dadX and the induction by L-Rhamnose to pure glycerol.]]
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[[File:Team-Bielefeld-Biosafety-System-TetORalive-FluorALL.jpg|600px|thumb|center|'''Figure 16:''' Characterization of the fluorescence of the Biosafety-System TetOR alive. The figure compares the wild tpye k-12 and the Biosafety-Strain K-12 ∆alr ∆dadX and the induction by L-Rhamnose to pure glycerol.]]
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[[File:Team-Bielefeld-Biosafety-System-TetORalive-sepzProductALL.jpg|600px|thumb|center|'''Figure 17:''' Specific production rate, calculated by equation (1).]]
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==='''Conclusion of the Results'''===
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[[File:Team-Bielefeld-Biosafety-System-TetORalive-Overview.jpg|600px|thumb|center|'''Figure 18:''' Comparision of the specific production rate of GFP in the with L-Rhamnose induced Biosafety-System TetOR alive, the uninduced Biosafety-System TetOR alive and the second part of the Biosafety-System (TetO-GFP only).]]
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Revision as of 12:22, 6 October 2013

Biosafety-System TetOR alive (TetR)

Biosafety-System TetOR alive

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1156
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1080
    Illegal BamHI site found at 1782
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1198
    Illegal AgeI site found at 1498
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 955
    Illegal BsaI.rc site found at 2774
Figure 12: Characterization of the bacterial growth of the TetO promoter with GFP (BBa_E0040) in the Biosafety-Strain K-12 ∆alr ∆dadX. The M9 media was supplemented with 5 mM D-alanine. It can be seen, that the bacteria grow faster on M9 minimal media glucose than on M9 minimal media glycerol.
Figure 13: Characterization of the fluorescence of TetO promoter with GFP (BBa_E0040) in the Biosafety-Strain K-12 ∆alr ∆dadX. The bacteria were cultivated on M9 minimal media with 5 mM D-alanine supplemented..


Figure 14: Specific growth rate of GFP behind the TetO promoter by the use of different carbon sources.

Characterization of the Biosafety-System TetOR alive

Figure 15: Characterization of the bacterial growth of the Biosafety-System on M9 minimal media glycerol. The figure compares the wild tpye K-12 and the Biosafety-Strain K-12 ∆alr ∆dadX and the induction by L-Rhamnose to pure glycerol.


Figure 16: Characterization of the fluorescence of the Biosafety-System TetOR alive. The figure compares the wild tpye k-12 and the Biosafety-Strain K-12 ∆alr ∆dadX and the induction by L-Rhamnose to pure glycerol.
Figure 17: Specific production rate, calculated by equation (1).


Conclusion of the Results

File:Team-Bielefeld-Biosafety-System-TetORalive-Overview.jpg