Difference between revisions of "Part:BBa K1172912"
 
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Part 1 of the Biosystem M (pRha TetR alr)
 
Part 1 of the Biosystem M (pRha TetR alr)
  
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===Usage and Biology===
 
===Usage and Biology===
  
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K1172912 parameters</partinfo>
 
<partinfo>BBa_K1172912 parameters</partinfo>
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Latest revision as of 04:57, 5 October 2013

Part 1 of the Biosystem TetOR alive (pRha TetR alr)

Part 1 of the Biosystem M (pRha TetR alr)


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1156
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1080
    Illegal BamHI site found at 1782
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1198
    Illegal AgeI site found at 1498
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 955

Results


Characterization of the tetracycline operator TetO

Figure 12: Characterization of the bacterial growth of the TetO promoter with GFP (BBa_E0040) in the Biosafety-Strain K-12 ∆alr ∆dadX. The M9 media was supplemented with 5 mM D-alanine. It can be seen, that the bacteria grow faster on M9 minimal media glucose than on M9 minimal media glycerol.
Figure 13: Characterization of the fluorescence of TetO promoter with GFP (BBa_E0040) in the Biosafety-Strain K-12 ∆alr ∆dadX. The bacteria were cultivated on M9 minimal media with 5 mM D-alanine supplemented..


Figure 14: Specific growth rate of GFP behind the TetO promoter by the use of different carbon sources.

Characterization of the Biosafety-System TetOR alive

Figure 15: Characterization of the bacterial growth of the Biosafety-System on M9 minimal media glycerol. The figure compares the wild tpye K-12 and the Biosafety-Strain K-12 ∆alr ∆dadX and the induction by L-Rhamnose to pure glycerol.


Figure 16: Characterization of the fluorescence of the Biosafety-System TetOR alive. The figure compares the wild tpye k-12 and the Biosafety-Strain K-12 ∆alr ∆dadX and the induction by L-Rhamnose to pure glycerol.
Figure 17: Specific production rate, calculated by equation (1).


Conclusion of the Results

File:Team-Bielefeld-Biosafety-System-TetORalive-Overview.jpg


References

Saenger W. et al. (2000):Der Tetracyclin-Repressor – das Musterbeispiel für einen biologischen Schalter, In:[http://onlinelibrary.wiley.com/doi/10.1002/1521-3757%2820000616%29112:12%3C2122::AID-ANGE2122%3E3.0.CO;2-8/abstract Angewandte Chemie Volume 112, Issue 12, pages 2122–2133]

Functional Parameters