Difference between revisions of "Part:BBa K1073026"

(→‎Usage and Biology)
 
Line 2: Line 2:
 
<partinfo>BBa_K1073026 short</partinfo>
 
<partinfo>BBa_K1073026 short</partinfo>
  
−
The device consists of a strong promoter (<partinfo>BBa_J23100</partinfo>) combined with RBS (<partinfo>BBa_B0032</partinfo>) and the encoding region of the chromoprotein cjBlue. This results in a constitutively high expression of cjBlue.  
+
This device is an enhanced construct of 2012 Uppsala's <partinfo>BBa_K592011</partinfo>. It consists of a strong promoter (<partinfo>BBa_J23100</partinfo>) combined with RBS (<partinfo>BBa_B0032</partinfo>) and the encoding region of the chromoprotein cjBlue (<partinfo>BBa_K592011</partinfo>). This results in a constitutively high expression of cjBlue.  
  
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 18:01, 4 October 2013

Constitutively expressed chromoprotein cjBlue

This device is an enhanced construct of 2012 Uppsala's BBa_K592011. It consists of a strong promoter (BBa_J23100) combined with RBS (BBa_B0032) and the encoding region of the chromoprotein cjBlue (BBa_K592011). This results in a constitutively high expression of cjBlue.

Usage and Biology

During cultivation, the chromoprotein cjBlue is expressed and stored intracellularly. The greenish color of the protein can be seen by the naked eye, thus the chromoprotein can be used as a reporter to distinguish cells easily.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]