Difference between revisions of "Part:BBa K1073022"
Line 2: Line 2:
 
<partinfo>BBa_K1073022 short</partinfo>
 
<partinfo>BBa_K1073022 short</partinfo>
  
−
This device is an enhanced construct of 2012 Uppsala's <partinfo>BBa_K592012</partinfo>. It consists of a strong promoter (<partinfo>BBa_J23100</partinfo>) combined with a RBS (<partinfo>BBa_B0032</partinfo>) and the encoding region of the chromoprotein eforRed <partinfo>BBa_K592012</partinfo>. This results in a constitutively high expression of eforRed.  
+
This device is an enhanced construct of 2012 Uppsala's <partinfo>BBa_K592012</partinfo>. It consists of a strong promoter (<partinfo>BBa_J23100</partinfo>) combined with a RBS (<partinfo>BBa_B0032</partinfo>) and the encoding region of the chromoprotein eforRed (<partinfo>BBa_K592012</partinfo>). This results in a constitutively high expression of eforRed.  
  
  

Revision as of 17:58, 4 October 2013

Constitutively expressed chromoprotein eforRed

This device is an enhanced construct of 2012 Uppsala's BBa_K592012. It consists of a strong promoter (BBa_J23100) combined with a RBS (BBa_B0032) and the encoding region of the chromoprotein eforRed (BBa_K592012). This results in a constitutively high expression of eforRed.


Usage and Biology

During cultivation, the chromoprotein eforRed is expressed and stored intracellularly. The pink color of the protein can be seen by the naked eye, thus the chromoprotein can be used as a reporter to distinguish cells easily.

Expressed chromoproteins.png EforRed pellet.png

iGEM Team Braunschweig 2013:

Left: Liquid cultures of E. coli XL1 expressing chromoproteins amilGFP (BBa_K1073024), eforRed and aeBlue (BBa_K1073020).

Right: Cell pellet of E. coli XL1 expressing eforRed.


The pink color of eforRed can be seen by the naked eye. The chromoprotein also fluoresce when excited. Thus, it can be used to identify cells with fluorescence microscopy or flow cytometry. For further information as well as absorption and emission spectra see experience page.


Fluorescence.JPG

iGEM Team Braunschweig 2013: Supernatant after cell disruption containing chromoproteins of eforRed and amilGFP (BBa_K1073024). Both chromoproteins fluoresce when excited by UV-light.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]