Difference between revisions of "Part:BBa K1022109"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1022109 short</partinfo>
 
<partinfo>BBa_K1022109 short</partinfo>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1022109 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1022109 SequenceAndFeatures</partinfo>
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==Characterization==
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'''For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch  Killswitch] on TU Delft iGEM13 Wiki'''
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===Suffix Improvement===
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'''Introduction:'''
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The construct BBa_K1022109 is made by ligating the existing biobrick [https://parts.igem.org/wiki/index.php?title=Part:BBa_K112808  BBa_K112808] in pSB1C3 backbone in order to correct the suffix, as it was observed that the suffix in the existing part ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K112808  BBa_K112808]) didnot have a '''Not I''' site.
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This correction is useful for future cloning and sequence analysis. [https://parts.igem.org/Assembly_standard_10 RFC10 standard] is thus fixed due to this.
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For a detailed characterization of the more improved part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1022114  BBa_K1022114] with a pT7 promoter, please visit [http://2013.igem.org/Team:TU-Delft/Killswitch  Killswitch] on TU Delft iGEM13 Wiki.
  
  

Latest revision as of 12:19, 4 October 2013

Lysis device with corrected Suffix

This part is an improvement of the Bio-brick Bba_K112808. It has a corrected suffix from the earlier version.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1350
    Illegal NheI site found at 1373
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 955
    Illegal AgeI site found at 1025
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1606

Characterization

For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch Killswitch] on TU Delft iGEM13 Wiki

Suffix Improvement

Introduction:

The construct BBa_K1022109 is made by ligating the existing biobrick BBa_K112808 in pSB1C3 backbone in order to correct the suffix, as it was observed that the suffix in the existing part (BBa_K112808) didnot have a Not I site.

This correction is useful for future cloning and sequence analysis. RFC10 standard is thus fixed due to this.

For a detailed characterization of the more improved part BBa_K1022114 with a pT7 promoter, please visit [http://2013.igem.org/Team:TU-Delft/Killswitch Killswitch] on TU Delft iGEM13 Wiki.