Difference between revisions of "PBAD SPL"

Revision as of 11:02, 4 October 2013

Here the DTU iGEM team will present their work on characterizing a SPL of pBAD promoters.

List of sequences:

DTU pBAD SPL

Identifier	Sequence^a	Induced Strength	Leakyness	Inducibility
Col. 2	CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA	14.2	0.203	69.9
Col. 3	CTGACGTAATCATACCGCCGAAAGTATTATCATTA	16.6	0.426	39
Col. 4	CTGACGCGACAACATTGCGTCCTATAAAATGCCGA	14.2	0.345	41
Col. 5	CTGACGGCCCGCCCCCGATGCGCATAAAATACCAA	17.7	0.424	33
Col. 12	CTGACGCGTATCGCGAGCGGGCGTTATTATACGCA	13.6	0.414	38.8
Col. 10	CTGACGCCCCGAATCAGTAGTATTTATTATCTAGA	18	0.462	30.4
Col. 9	CTGACGCCACCCCCCCCCCCGGCGTATAATTCCCA	16.1	0.530	63.4
Col. 8	CTGACGCCCTACCGCTCGCCCCCCTATTATACCCA	13.4	0.211	113.6
Col. 13	CTGACGCCCCCCCCGTCCACCCCCTAATATCCCGA	10.9	0.096	65.8
Col. 15	CTGACGTGCGCCTGCCGTCCAAAGTAATATCCTTA	15.5	0.235	37.8
Col. 18	CTGACGCCCCGAATCAGTAGTATTTATTATCTAGA	19.8	0.524	57
Col. 19	CTGACGCTCGCACCGGCGACCCGATAATATCCATA	18.0	0.315	23.6
Col. 31	CTGACGCCCCCCCTCCCCCGACCTAAAATATCCAG	3.8	0.161	49.2
Col. 29	CTGACGCCGCTGCACCCGTCCCCCTATTATAGCAA	14.8	0.301	47.2
Col. 26	CTGACGCCGATCCTATCTCCCTATTAAAATTTTTA	13.8	0.292	36.1
Col. 22	CTGACGCCCCGAATCAGTAGTATTTATTATCTAGA	16.8	0.466	14.3
Col. 33	CTGACGCCAGCTTCCTACTTCAAATATAATCCGTA	14.4	1.01	65.9
Col. 34	CTGACGTCCCTCTTCCGCGCCCCCTAAAATACCCA	12.4	0.187	20.7
Col. 35	CTGACGCCCAACCCGACACAGCGATATAATAATGA	18.4	0.89
BBa_J23118	ttgacggctagctcagtcctaggtattgtgctagc	0.56

@@ Line 8: / Line 8: @@
 ! style="background:#AAAAAA; color:black"  |Sequence<sup>a</sup>
 ! style="background:#AAAAAA; color:black"  |Induced Strength
-! colspan="2" style="background:#AAAAAA; color:black"  |Leakyness
+! style="background:#AAAAAA; color:black"  |Leakyness
+! colspan="2" style="background:#AAAAAA; color:black"  |Inducibility
 |-
@@ Line 15: / Line 16: @@
 |align="center"|14.2
 |align="center"|0.203
+|align="center"|69.9
 |-
 |align="center"|Col. 3
@@ Line 20: / Line 22: @@
 |align="center"|16.6
 |align="center"|0.426
+|align="center"|39
 |-
 |align="center"|Col. 4
@@ Line 25: / Line 28: @@
 |align="center"|14.2
 |align="center"|0.345
+|align="center"|41
 |-
 |align="center"|Col. 5
@@ Line 30: / Line 34: @@
 |align="center"|17.7
 |align="center"|0.424
+|align="center"|33
 |-
 |align="center"|Col. 12
@@ Line 35: / Line 40: @@
 |align="center"|13.6
 |align="center"|0.414
+|align="center"|38.8
 |-
 |align="center"|Col. 10
@@ Line 40: / Line 46: @@
 |align="center"|18
 |align="center"|0.462
+|align="center"|30.4
 |-
 |align="center"|Col. 9
@@ Line 45: / Line 52: @@
 |align="center"|16.1
 |align="center"|0.530
+|align="center"|63.4
 |-
 |align="center"|Col. 8
@@ Line 50: / Line 58: @@
 |align="center"|13.4
 |align="center"|0.211
+|align="center"|113.6
 |-
 |align="center"|Col. 13
@@ Line 55: / Line 64: @@
 |align="center"|10.9
 |align="center"|0.096
+|align="center"|65.8
 |-
 |align="center"|Col. 15
@@ Line 60: / Line 70: @@
 |align="center"|15.5
 |align="center"|0.235
+|align="center"|37.8
 |-
 |align="center"|Col. 18
@@ Line 65: / Line 76: @@
 |align="center"|19.8
 |align="center"|0.524
+|align="center"|57
 |-
 |align="center"|Col. 19
@@ Line 70: / Line 82: @@
 |align="center"|18.0
 |align="center"|0.315
+|align="center"|23.6
 |-
 |align="center"|Col. 31
@@ Line 75: / Line 88: @@
 |align="center"|3.8
 |align="center"|0.161
+|align="center"|49.2
 |-
 |align="center"|Col. 29
@@ Line 80: / Line 94: @@
 |align="center"|14.8
 |align="center"|0.301
+|align="center"|47.2
 |-
 |align="center"|Col. 26
@@ Line 85: / Line 100: @@
 |align="center"|13.8
 |align="center"|0.292
+|align="center"|36.1
 |-
 |align="center"|Col. 22
@@ Line 90: / Line 106: @@
 |align="center"|16.8
 |align="center"|0.466
+|align="center"|14.3
 |-
 |align="center"|Col. 33
@@ Line 95: / Line 112: @@
 |align="center"|14.4
 |align="center"|1.01
+|align="center"|65.9
 |-
 |align="center"|Col. 34
@@ Line 100: / Line 118: @@
 |align="center"|12.4
 |align="center"|0.187
+|align="center"|20.7
 |-
 |align="center"|Col. 35