Difference between revisions of "PBAD SPL"

(DTU pBAD SPL)
Line 7: Line 7:
 
! style="background:#AAAAAA; color:black"  |Identifier
 
! style="background:#AAAAAA; color:black"  |Identifier
 
! style="background:#AAAAAA; color:black"  |Sequence<sup>a</sup>
 
! style="background:#AAAAAA; color:black"  |Sequence<sup>a</sup>
! colspan="2" style="background:#AAAAAA; color:black"  |Induced Strength<sup>b</sup>
+
! style="background:#AAAAAA; color:black"  |Induced Strength
! colspan="2" style="background:#AAAAAA; color:black"  |Leakyness<sup>b</sup>
+
! colspan="2" style="background:#AAAAAA; color:black"  |Leakyness
 +
 
 
|-
 
|-
 
|align="center"|Col. 2
 
|align="center"|Col. 2
 
|<font face="Courier" size="+1">CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA</font>
 
|<font face="Courier" size="+1">CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA</font>
 
|align="center"|14.2
 
|align="center"|14.2
|align="center"|14.2
+
|align="center"|0.203
 
|-
 
|-
 
|align="center"|Col. 3
 
|align="center"|Col. 3
 
|<font face="Courier" size="+1">CTGACGTAATCATACCGCCGAAAGTATTATCATTA</font>
 
|<font face="Courier" size="+1">CTGACGTAATCATACCGCCGAAAGTATTATCATTA</font>
 
|align="center"|16.6
 
|align="center"|16.6
|align="center"|14.2
+
|align="center"|0.426
 
|-
 
|-
 
|align="center"|Col. 4
 
|align="center"|Col. 4
 
|<font face="Courier" size="+1">CTGACGCGACAACATTGCGTCCTATAAAATGCCGA</font>
 
|<font face="Courier" size="+1">CTGACGCGACAACATTGCGTCCTATAAAATGCCGA</font>
 
|align="center"|14.2
 
|align="center"|14.2
|align="center"|14.2
+
|align="center"|0.345
 
|-
 
|-
 
|align="center"|<partinfo>J23102</partinfo>
 
|align="center"|<partinfo>J23102</partinfo>
Line 93: Line 94:
 
|align="center"|0.56
 
|align="center"|0.56
 
|-
 
|-
|}
 
 
 
 
 
 
 
 
 
 
{| class="wikitable"
 
|-
 
! Mutant name !! Sequence
 
|-
 
| Mut. 1 || CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA
 
|-
 
| Mut. 2 || CTGACGTAATCATACCGCCGAAAGTATTATCATTA
 
|-
 
| Mut. 3 || CTGACGCGACAACATTGCGTCCTATAAAATGCCGA
 
 
|}
 
|}

Revision as of 10:39, 4 October 2013

Here the DTU iGEM team will present their work on characterizing a SPL of pBAD promoters.

List of sequences:

DTU pBAD SPL

Identifier Sequencea Induced Strength Leakyness
Col. 2 CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA 14.2 0.203
Col. 3 CTGACGTAATCATACCGCCGAAAGTATTATCATTA 16.6 0.426
Col. 4 CTGACGCGACAACATTGCGTCCTATAAAATGCCGA 14.2 0.345
BBa_J23102 ttgacagctagctcagtcctaggtactgtgctagc 0.86
BBa_J23103 ctgatagctagctcagtcctagggattatgctagc 0.01
BBa_J23104 ttgacagctagctcagtcctaggtattgtgctagc 0.72
BBa_J23105 tttacggctagctcagtcctaggtactatgctagc 0.24
BBa_J23106 tttacggctagctcagtcctaggtatagtgctagc 0.47
BBa_J23107 tttacggctagctcagccctaggtattatgctagc 0.36
BBa_J23108 ctgacagctagctcagtcctaggtataatgctagc 0.51
BBa_J23109 tttacagctagctcagtcctagggactgtgctagc 0.04
BBa_J23110 tttacggctagctcagtcctaggtacaatgctagc 0.33
BBa_J23111 ttgacggctagctcagtcctaggtatagtgctagc 0.58
BBa_J23112 ctgatagctagctcagtcctagggattatgctagc 0.00
BBa_J23113 ctgatggctagctcagtcctagggattatgctagc 0.01
BBa_J23114 tttatggctagctcagtcctaggtacaatgctagc 0.10
BBa_J23115 tttatagctagctcagcccttggtacaatgctagc 0.15
BBa_J23116 ttgacagctagctcagtcctagggactatgctagc 0.16
BBa_J23117 ttgacagctagctcagtcctagggattgtgctagc 0.06
BBa_J23118 ttgacggctagctcagtcctaggtattgtgctagc 0.56