Difference between revisions of "Part:BBa K1022110"

 
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<partinfo>BBa_K1022110 short</partinfo>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1022110 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1022110 SequenceAndFeatures</partinfo>
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===Characterization===
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'''For more info, visit [http://2013.igem.org/Team:TU-Delft/PeptideCharacterization  TU Delft iGEM13 Wiki]'''
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'''Peptide Characterization:'''
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An important part of the project TU Delft iGEM13 is inhibition of growth or killing of bacteria with the use of antimicrobial peptides (AMPs). In order to get an idea of the toxicity of our peptides we conducted several minimal inhibiting concentration (MIC) experiments. These MIC measurements where done on ''E. coli'', ''B. subtilis'' and ''S. delphini'', with the first as representative of our Gram-negative expression host, the second for the Gram-positive targets and the last for our specific target.
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'''MIC determination:'''
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The MIC of Maximin H5 on ''S. delphini, B. subtilis and E. coli'' were done according to the protocol described [http://2013.igem.org/Team:TU-Delft/PeptideCharacterization here].
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Maximin H5 did not give a measurable reduction in growth below 40µM (Figure 2), making us decide not to proceed testing, as modeling showed it was not possible to reach these concentrations through expression in ''E.coli''.
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[[Image:Figure2 Maximin TUD.jpg|800px|center]] Figure 1: MIC of Maximin H5 on ''S. delphini''
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'''For Other peptides and their characterization please visit [http://2013.igem.org/Team:TU-Delft/PeptideCharacterization Peptide characterization] on TU Delft iGEM13 Wiki'''
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==Peptide Production==
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'''For more info about the production of the peptide Maximin H5, visit [http://2013.igem.org/Team:TU-Delft/Peptides#SUMO  Peptide production] on TU Delft iGEM13 Wiki.
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Also check the construct used for the peptide production [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1022101  BBa_K1022101] and more characterization.''
  
  

Revision as of 12:12, 3 October 2013

PT7: RBS: Maximin H5: His 6

This bio-brick contains the promoter pT7 followed by Maximin H5 peptide with a His-6 tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

For more info, visit [http://2013.igem.org/Team:TU-Delft/PeptideCharacterization TU Delft iGEM13 Wiki]

Peptide Characterization:

An important part of the project TU Delft iGEM13 is inhibition of growth or killing of bacteria with the use of antimicrobial peptides (AMPs). In order to get an idea of the toxicity of our peptides we conducted several minimal inhibiting concentration (MIC) experiments. These MIC measurements where done on E. coli, B. subtilis and S. delphini, with the first as representative of our Gram-negative expression host, the second for the Gram-positive targets and the last for our specific target.

MIC determination:

The MIC of Maximin H5 on S. delphini, B. subtilis and E. coli were done according to the protocol described [http://2013.igem.org/Team:TU-Delft/PeptideCharacterization here]. Maximin H5 did not give a measurable reduction in growth below 40µM (Figure 2), making us decide not to proceed testing, as modeling showed it was not possible to reach these concentrations through expression in E.coli.

Figure2 Maximin TUD.jpg
Figure 1: MIC of Maximin H5 on S. delphini

For Other peptides and their characterization please visit [http://2013.igem.org/Team:TU-Delft/PeptideCharacterization Peptide characterization] on TU Delft iGEM13 Wiki

Peptide Production

For more info about the production of the peptide Maximin H5, visit [http://2013.igem.org/Team:TU-Delft/Peptides#SUMO Peptide production] on TU Delft iGEM13 Wiki.

Also check the construct used for the peptide production BBa_K1022101 and more characterization.