Difference between revisions of "Part:BBa K1150011"
| Line 25: | Line 25: | ||
Because we could not found the SV40 promotor in the parts registry, we decided to provide one to the following iGEM teams. This enables the teams to choose from differently strong mammalian promotors – dependent of the individual intention. | Because we could not found the SV40 promotor in the parts registry, we decided to provide one to the following iGEM teams. This enables the teams to choose from differently strong mammalian promotors – dependent of the individual intention. | ||
| − | We tested both promotors (BBa_K1150011 [SV40], BBa_K747096 [CMV]) | + | We tested our own devices with both promotors (BBa_K1150011 [SV40], BBa_K747096 [CMV]). For further information please take a look at BBa_K1150019 and BBa_K1150020. |
In an earlier study CMV and SV40 promoter were tested in different B and T cell as well as in two non-lymphoid cell. In all cell lines the CMV promoter construct was higher expressed than that of SV40 [Zarrin et al., 1999]. | In an earlier study CMV and SV40 promoter were tested in different B and T cell as well as in two non-lymphoid cell. In all cell lines the CMV promoter construct was higher expressed than that of SV40 [Zarrin et al., 1999]. | ||
Revision as of 21:28, 30 September 2013
SV40 promoter
| SV40 | |
|---|---|
| Function | promotor |
| Use in | Mammalian cells |
| RFC standard | RFC 10 |
| Backbone | pSB1C3 |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
SV40 promotor is a viral promotor derived from Simian virus 40.
Because we could not found the SV40 promotor in the parts registry, we decided to provide one to the following iGEM teams. This enables the teams to choose from differently strong mammalian promotors – dependent of the individual intention. We tested our own devices with both promotors (BBa_K1150011 [SV40], BBa_K747096 [CMV]). For further information please take a look at BBa_K1150019 and BBa_K1150020.
In an earlier study CMV and SV40 promoter were tested in different B and T cell as well as in two non-lymphoid cell. In all cell lines the CMV promoter construct was higher expressed than that of SV40 [Zarrin et al., 1999].
In comparison to the cellular promoter VV1 SV40 showed similar activities. Hence with an SV40 promotor one can gain a more physiologic control for reporter assays compared to CMV.
ZARRIN, A.; MALKIN, L.; FONG, I.; LUK, K.; GHOSE, A.; BERINSTEIN, N. (1999). Comparison of CMV, RSV, SV40 viral and VV1 cellular promoters in B and T lymphoid and non-lymphoid cell lines. Biochimica et Biophysica Acta 1446: 135-139.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]