Difference between revisions of "Part:BBa K1060003"

Revision as of 09:03, 30 September 2013

Methyl Salicylate Production

KU_Leuven 2013 requested the BBa_J45700 brick from MIT 2006 and recloned into the pSB1C3 standard plasmid backbone. For the assembly of this part we refer to the original MIT brick BBa_J45700 => "We assembled a complete wintergreen odor biosynthetic system (BBa_J45700) by combining the salicylic acid (BBa_J45320) and wintergreen odor generators (BBa_J45120). BBa_J45700 catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor."

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 744
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1689
Illegal NgoMIV site found at 2093
Illegal NgoMIV site found at 2218
Illegal NgoMIV site found at 2229
Illegal NgoMIV site found at 2510
Illegal NgoMIV site found at 2978
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 285

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1060003 short</partinfo>
-KU_Leuven 2013 requested the BBa_J45700 brick from MIT 2006 and recloned into the pSB1C3 standard plasmid backbone. For the assembly of this part we refer to the original MIT brick BBa_J45700 => "We assembled a complete wintergreen odor biosynthetic system (BBa_J45700) by combining the salicylic acid (BBa_J45320) and wintergreen odor generators (BBa_J45120). BBa_J45700 catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor."
+KU_Leuven 2013 requested the <partinfo>BBa_J45700</partinfo> brick from MIT 2006 and recloned into the pSB1C3 standard plasmid backbone. For the assembly of this part we refer to the original MIT brick <partinfo>BBa_J45700</partinfo> => "We assembled a complete wintergreen odor biosynthetic system (<partinfo>BBa_J45700</partinfo>) by combining the salicylic acid (<partinfo>BBa_J45320</partinfo>) and wintergreen odor generators (<partinfo>BBa_J45120</partinfo>). <partinfo>BBa_J45700</partinfo> catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor."
 <!-- Add more about the biology of this part here