Difference between revisions of "Part:BBa K1127018"
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<partinfo>BBa_K1127018 short</partinfo> | <partinfo>BBa_K1127018 short</partinfo> | ||
− | GFP generator is added | + | GFP generator is added to monitor activity of the switch BBa_K1127016 [https://parts.igem.org/Part:BBa_K1127016]. GFP is expressed when the switch is OFF (no quorum sensing signal AHL), and vice versa. |
==Figures== | ==Figures== |
Revision as of 22:20, 29 September 2013
QS and gold sensing part for chracterisation
GFP generator is added to monitor activity of the switch BBa_K1127016 [1]. GFP is expressed when the switch is OFF (no quorum sensing signal AHL), and vice versa.
Figures
The part was transformed into E. coli strain DH5 alpha. The bacteria were streaked onto LB agar supplemented with chloramphenicol and Xgal.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1747
Illegal NheI site found at 1770 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3565
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 701
Illegal AgeI site found at 880 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2808
Illegal BsaI.rc site found at 3714
Illegal BsaI.rc site found at 4461