Difference between revisions of "Part:BBa K1150063"
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right" | {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right" | ||
− | ! colspan="2" style="background:#FFBF00;"| | + | ! colspan="2" style="background:#FFBF00;"|HA-NLS-Cas9-Linker |
|- | |- | ||
|'''Function''' | |'''Function''' | ||
− | | | + | |A new effector can be fused to |
− | + | this Cas9 intermediate part | |
− | + | ||
|- | |- | ||
|'''Use in''' | |'''Use in''' | ||
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|- | |- | ||
|'''RFC standard''' | |'''RFC standard''' | ||
− | |[https://parts.igem.org/Help:Assembly_standard_25 RFC 25] | + | |Front [https://parts.igem.org/Help:Assembly_standard_10 RFC 10] & back [https://parts.igem.org/Help:Assembly_standard_25 RFC 25] |
|- | |- | ||
|'''Backbone''' | |'''Backbone''' |
Revision as of 00:00, 29 September 2013
HA-NLS-Cas9-Linker 3 AA
.
HA-NLS-Cas9-Linker | |
---|---|
Function | A new effector can be fused to
this Cas9 intermediate part |
Use in | Mammalians |
RFC standard | Front RFC 10 & back RFC 25 |
Backbone | pSB1C3 |
Organism | Arabidopsis thaliana |
Source | AG Weber, Freiburg |
Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
Biology and Usage
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 312
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Literature