Difference between revisions of "Part:BBa K1150063"

Line 4: Line 4:
 
.
 
.
 
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
 
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
! colspan="2" style="background:#FFBF00;"|COP1
+
! colspan="2" style="background:#FFBF00;"|HA-NLS-Cas9-Linker
 
|-
 
|-
 
|'''Function'''
 
|'''Function'''
|interaction partner of
+
|A new effector can be fused to
 
+
this Cas9 intermediate part
UVR8,reacts to UVB light
+
 
|-
 
|-
 
|'''Use in'''
 
|'''Use in'''
Line 15: Line 14:
 
|-
 
|-
 
|'''RFC standard'''
 
|'''RFC standard'''
|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
+
|Front [https://parts.igem.org/Help:Assembly_standard_10 RFC 10] & back [https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
 
|-
 
|-
 
|'''Backbone'''
 
|'''Backbone'''

Revision as of 00:00, 29 September 2013

HA-NLS-Cas9-Linker 3 AA

.

HA-NLS-Cas9-Linker
Function A new effector can be fused to

this Cas9 intermediate part

Use in Mammalians
RFC standard Front RFC 10 & back RFC 25
Backbone pSB1C3
Organism Arabidopsis thaliana
Source AG Weber, Freiburg
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

Biology and Usage

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 312
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Literature