Difference between revisions of "Part:BBa K1073026"
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| − | During cultivation, the chromoprotein cjBlue is expressed and stored intracellularly. The greenish color of the protein can be seen by naked eye, thus you can use the chromoprotein as a reporter | + | During cultivation, the chromoprotein cjBlue is expressed and stored intracellularly. The greenish color of the protein can be seen by naked eye, thus you can use the chromoprotein as a reporter to distinguish cells easily. |
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Revision as of 12:47, 28 September 2013
Constitutively expressed chromoprotein cjBlue
The device consists of a strong promoter (BBa_J23100) combined with RBS (BBa_B0032) and the encoding region of the chromoprotein cjBlue. This results in a constitutively high expression of cjBlue.
Usage and Biology
During cultivation, the chromoprotein cjBlue is expressed and stored intracellularly. The greenish color of the protein can be seen by naked eye, thus you can use the chromoprotein as a reporter to distinguish cells easily.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]