Difference between revisions of "Part:BBa K1189021"

Revision as of 01:20, 28 September 2013

TALE-A ferritin di-subunit direct fusion w/ hisTag under lacI promoter, and linker

This part was created by the fusion of the heavy and light ferritin subunits (BBa_K1189025, BBa_K1189024) to form the fusion ferritin subunit. The lacI promoter (BBa_J04500), double terminator (BBa_B0015) and his-tag was also added in order to induce protein expression and allow purification. This part is similar to BBa_K1189018, but instead of the E-coil, it is replaced by TALA(BBa_K1189004). This part was primarily used as a backup in the case the coiled-coil system was ineffective at binding TALEs to the ferritin scaffold.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2654
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 3656

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 <partinfo>BBa_K1189021 short</partinfo>
-This part was created by the fusion of the heavy and light ferritin subunits (PARTSTS) to form the form the fusion subunit. The lacI promoter (PART), double terminator (PART) and his tag was also added in order to induce protein expression and allow purification. This part is similar to ____ PART, but instead of the E-coil, it is replaced by TALB(PART). This part was primarily used as a backup in the case our coiled-coil systems did not bind to eachother. It was intended to have the same functions as _____ PART___, but with the additional DNA binding capabilities given by TALB.
+This part was created by the fusion of the heavy and light ferritin subunits (<partinfo>BBa_K1189025</partinfo>, <partinfo>BBa_K1189024</partinfo>) to form the fusion ferritin subunit. The lacI promoter (<partinfo>BBa_J04500</partinfo>), double terminator (<partinfo>BBa_B0015</partinfo>) and his-tag was also added in order to induce protein expression and allow purification. This part is similar to <partinfo>BBa_K1189018</partinfo>, but instead of the E-coil, it is replaced by TALA(<partinfo>BBa_K1189004</partinfo>). This part was primarily used as a backup in the case the coiled-coil system was ineffective at binding TALEs to the ferritin scaffold.
 <!-- Add more about the biology of this part here