Difference between revisions of "Part:BBa K1124106:Design"
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===References=== | ===References=== | ||
Yoo, S. M., Na, D., & Lee, S. Y. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature protocols, 8(9), 1694-1707. | Yoo, S. M., Na, D., & Lee, S. Y. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature protocols, 8(9), 1694-1707. | ||
Latest revision as of 23:59, 27 September 2013
plambda-sRNA(anti-tyrR)-plambda-sRNA (anti-csrA) (tyrosine synthesis device)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In this composite part, we chose the phage lambda pR promoter(Part:BBa_R0051) as the promoter of sRNA because the transcriptional start site of pR promoter was well determined, and its operator site is embedded within the pR promoter. These characteristics strongly suggest that no dummy nucleotides will be attached to the sRNA.
References
Yoo, S. M., Na, D., & Lee, S. Y. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature protocols, 8(9), 1694-1707.