Difference between revisions of "Part:BBa K1031111"

Latest revision as of 21:33, 27 September 2013

J23109-CapR-Terminator

Characterization

In order to fine-tune the performance of CapR biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031111 is composed of three elements, the constitutive Pc promoter J23109, coding sequence of CapR and terminator B0015. (Fig 1)

Fig 1 Construction of biosensor circuit Po/J23109-CapR. The orange arrowheads represent promoters, RBS is shown as a green oval, and the red circle stands for B0015 terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 147
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 344
Illegal NgoMIV site found at 625
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 851
Illegal SapI.rc site found at 1508

@@ Line 12: / Line 12: @@
 <html>
 <img src="https://static.igem.org/mediawiki/igem.org/thumb/2/2f/Peking2013_part_109-CapR-TT.png/800px-Peking2013_part_109-CapR-TT.png" style="width:600px; margin-left:170px" />
-<p style="text-align:center"><b>Fig 1</b> Construction of biosensor circuit ''Po''/J23109-CapR. The orange arrowheads represent promoters, RBS is shown as a green oval, and the red circle stands for B0015 terminator.
+<p style="text-align:center"><b>Fig 1</b> Construction of biosensor circuit <i>Po</i>/J23109-CapR. The orange arrowheads represent promoters, RBS is shown as a green oval, and the red circle stands for B0015 terminator.
 </html>