Difference between revisions of "Part:BBa K1031111"
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− | In order to fine-tune the performance of CapR biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031111 is composed of three elements, the constitutive ''Pc'' promoter | + | In order to fine-tune the performance of CapR biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031111 is composed of three elements, the constitutive ''Pc'' promoter <html><a href="https://parts.igem.org/Part:BBa_J23109">J23109</a></html>, coding sequence of CapR and terminator <html><a href="https://parts.igem.org/Part:BBa_B0015">B0015</a></html>. ('''Fig 1''') |
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− | <img src="https://static.igem.org/mediawiki/igem.org/thumb/2/2f/Peking2013_part_109-CapR-TT.png/800px-Peking2013_part_109-CapR-TT.png" | + | <img src="https://static.igem.org/mediawiki/igem.org/thumb/2/2f/Peking2013_part_109-CapR-TT.png/800px-Peking2013_part_109-CapR-TT.png" style="width:600px; margin-left:170px" /> |
+ | <p style="text-align:center"><b>Fig 1</b> Construction of biosensor circuit ''Po''/J23109-CapR. The orange arrowheads represent promoters, RBS is shown as a green oval, and the red circle stands for B0015 terminator. | ||
</html> | </html> | ||
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Revision as of 21:33, 27 September 2013
J23109-CapR-Terminator
Characterization
In order to fine-tune the performance of CapR biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031111 is composed of three elements, the constitutive Pc promoter J23109, coding sequence of CapR and terminator B0015. (Fig 1)
Fig 1 Construction of biosensor circuit ''Po''/J23109-CapR. The orange arrowheads represent promoters, RBS is shown as a green oval, and the red circle stands for B0015 terminator.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 147
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 344
Illegal NgoMIV site found at 625 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 851
Illegal SapI.rc site found at 1508