Difference between revisions of "Part:BBa K1189018"
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<p><b>Team:Calgary</b> Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period. </p> | <p><b>Team:Calgary</b> Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period. </p> | ||
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− | <p> The resulting | + | <p> The resulting molecule can then catalyze the formation of radicals from hydrogen peroxide, which can then cause a colour change in substrates such as TMB or ABTS. |
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https://static.igem.org/mediawiki/2013/6/6c/UCalgary2013TRSubstratecolourpartspage.png | https://static.igem.org/mediawiki/2013/6/6c/UCalgary2013TRSubstratecolourpartspage.png |
Revision as of 08:04, 27 September 2013
Human ferritin di-subunit with E coil w/ LacI promoter
This part was created by fusing the heavy chain and light chains (BBa_K1189024 BBa_K1189025) of human ferritin together. It is expressed under the lacI promoter (BBa_J04500) and has a his-tag for protein purification. An E-coil (BBa_K1189011) is included in order to allow binding of parts containing the respective K-coil (BBa_K1189010).
This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.
Team:Calgary Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period.
The resulting molecule can then catalyze the formation of radicals from hydrogen peroxide, which can then cause a colour change in substrates such as TMB or ABTS.
Team:Calgary Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.
Something about scaffold…
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1289