Difference between revisions of "Part:BBa K1189018"
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<p><b>Figure 4.</b> Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period. </p>
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<p><b>Team:Calgary</b> Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period. </p>
 
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<p> The resulting molecuel can then catalyse the formation of radicals from hydrogen perxoide, which can then cause a colour change in substrates such as TMB or ABTS.
 
<p> The resulting molecuel can then catalyse the formation of radicals from hydrogen perxoide, which can then cause a colour change in substrates such as TMB or ABTS.
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<b>Figure 11.</b> Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.</p>
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<b>Team:Calgary</b> Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.</p>
 
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Something about scaffold…  
 
Something about scaffold…  

Revision as of 08:04, 27 September 2013

Human ferritin di-subunit with E coil w/ LacI promoter

This part was created by fusing the heavy chain and light chains (BBa_K1189024 BBa_K1189025) of human ferritin together. It is expressed under the lacI promoter (BBa_J04500) and has a his-tag for protein purification. An E-coil (BBa_K1189011) is included in order to allow binding of parts containing the respective K-coil (BBa_K1189010).

This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.


UCalgary2013TRPrussianblueferritinsynthesispartspage.png

Team:Calgary Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period.


The resulting molecuel can then catalyse the formation of radicals from hydrogen perxoide, which can then cause a colour change in substrates such as TMB or ABTS.
UCalgary2013TRSubstratecolourpartspage.png
Team:Calgary Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.


Something about scaffold…


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1289