Difference between revisions of "Part:BBa K1073020:Experience"

Revision as of 19:39, 26 September 2013

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Applications of BBa_K1073020

When aeBlue is expressed, the colonies on plates as well as the liquid culture become blue. The iGEM Team Braunschweig 2013 used this device to differentiate two different strains from one approach.

User Reviews

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iGEM Team Braunschweig 2013

The chromoprotein aeBlue exhibits a distinct blue color when expressed. In order to avoid absoption of the chomoproteins during measurement with the photometer the absorption and emission spectra were measured.

Braunschweig 2013: Absorption spectrum of chromoprotein aeBlue in a wavelength range of 400-800 nm.

Absorption maximum for aeBlue: 595 nm

Emission maximum for aeBlue: none (dark quencher)

Accordingly the photometer measurements for growth curves were carried out by 520 nm with respect to the chromoproteins amilGFP (BBa_K1073024) and eforRed (BBa_K1073022).

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 <I>iGEM Team Braunschweig 2013</I>
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-The chromoprotein aeBlue has a distinct blue color. In order to avoid absoption of the chomoproteins during measurement with the photometer, the absorption and emission spectra were measured.
+The chromoprotein aeBlue exhibits a distinct blue color when expressed. In order to avoid absoption of the chomoproteins during measurement with the photometer the absorption and emission spectra were measured.
 [[File:AeBlue.jpg |500px]]
-'''Braunschweig 2013:''' Absorptionspectrum of chromoprotein aeBlue in the wavelength range 400-800 nm.
+'''Braunschweig 2013:''' Absorption spectrum of chromoprotein aeBlue in a wavelength range of 400-800 nm.