Difference between revisions of "Part:BBa K1073011"
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<partinfo>BBa_K1073011 short</partinfo> | <partinfo>BBa_K1073011 short</partinfo> | ||
| − | This device is a combination of <partinfo>BBa_K1073001</partinfo> and <partinfo> | + | This device is a combination of <partinfo>BBa_K1073001</partinfo> and <partinfo>BBa_K1073000</partinfo> and mediates an inducible ampicillin resistance. It includes the inducible promoter of the rhl quorum sensing system of'' Pseudomonas aeruginosa'' followed by a RBS and the ampicillin resistance gene coding for beta-lactamase. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 14:49, 26 September 2013
Inducible ampicillin resistance cassette (regulated by RhlR + N-buturyl-HSL)
This device is a combination of BBa_K1073001 and BBa_K1073000 and mediates an inducible ampicillin resistance. It includes the inducible promoter of the rhl quorum sensing system of Pseudomonas aeruginosa followed by a RBS and the ampicillin resistance gene coding for beta-lactamase.
Usage and Biology
The promoter is activated in the presence of RhlR in combination with the autoinducer molecule N-buturyl-homoserine lactone produced by RhlI. The expression of the beta-lactamase can therefore be induced and tuned by supplying RhlR and N-buturyl-HSL.
Note that the promoter is leaking to a certain degree when not activated. For further information and experience on this device see BBa_K1073035.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]