Difference between revisions of "Part:BBa K1073011"

Revision as of 14:48, 26 September 2013

Inducible ampicillin resistance cassette (regulated by RhlR + N-buturyl-HSL)

This device is a combination of BBa_K1073001 and No part name specified with partinfo tag. and mediates an inducible ampicillin resistance. It includes the inducible promoter of the rhl quorum sensing system of Pseudomonas aeruginosa followed by a RBS and the ampicillin resistance gene coding for beta-lactamase.

Usage and Biology

The promoter is activated in the presence of RhlR in combination with the autoinducer molecule N-buturyl-homoserine lactone produced by RhlI. The expression of the beta-lactamase can therefore be induced and tuned by supplying RhlR and N-buturyl-HSL.

Note that the promoter is leaking to a certain degree when not activated. For further information and experience on this device see BBa_K1073035.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1073011 short</partinfo>
-This device is a combination of BBa_K1073001 and BB_K1073000 and mediates a inducible ampicillin resistance. It includes the inducible promoter of the rhl quorum sensing system of'' Pseudomonas aeruginosa'' followed by a RBS and the ampicillin resistance gene coding for beta-lactamase.
+This device is a combination of <partinfo>BBa_K1073001</partinfo> and <partinfo>BBa_1073000</partinfo> and mediates an inducible ampicillin resistance. It includes the inducible promoter of the rhl quorum sensing system of'' Pseudomonas aeruginosa'' followed by a RBS and the ampicillin resistance gene coding for beta-lactamase.
 ===Usage and Biology===
 The promoter is activated in the presence of RhlR in combination with the autoinducer molecule N-buturyl-homoserine lactone produced by RhlI. The expression of the beta-lactamase can therefore be induced and tuned by supplying RhlR and N-buturyl-HSL.
-Note that the promoter is leaking to a certain degree when not activated. For further information and experience on this device see BBa_K1073035.
+Note that the promoter is leaking to a certain degree when not activated. For further information and experience on this device see <partinfo>BBa_K1073035</partinfo>.