Difference between revisions of "Part:BBa K1045013:Design"
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===Design Notes===
 
===Design Notes===
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For cloning of BBa_K1045013, first, oligos for BBa_K1045012 were hybridized. The hybridization oligos were designed such that their double-stranded hybridization product would correspond to the sequence of BBa_K1045012 cut with EcoRI and SpeI at the prefix and suffix sites. Second, this hybridization product was ligated in front of the GFP generator part BBa_E0240 previously cut with EcoRI and XbaI.
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For cloning of [[Part:BBa_K1045013|BBa_K1045013]], first, oligos for [[Part:BBa_K1045012|BBa_K1045012]] were hybridized. The hybridization oligos were designed such that their double-stranded hybridization product would correspond to the sequence of [[Part:BBa_K1045012|BBa_K1045012]] cut with EcoRI and SpeI at the prefix and suffix sites. Second, this hybridization product was ligated in front of the GFP generator [[Part:BBa_E0240|BBa_E0240]] previously cut with EcoRI and XbaI.
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===Source===
 
===Source===

Revision as of 12:07, 25 September 2013

Promoter - DarR operator - GFP generator BBa_E0240


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 728


Design Notes

For cloning of BBa_K1045013, first, oligos for BBa_K1045012 were hybridized. The hybridization oligos were designed such that their double-stranded hybridization product would correspond to the sequence of BBa_K1045012 cut with EcoRI and SpeI at the prefix and suffix sites. Second, this hybridization product was ligated in front of the GFP generator BBa_E0240 previously cut with EcoRI and XbaI.

Source

To be continued

References