Difference between revisions of "Part:BBa K1114032"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1114032 short</partinfo>
 
<partinfo>BBa_K1114032 short</partinfo>
  
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===Design Notes===
 
 
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<html>
 
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_I13453 with GB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_I13453">BBa_I13453</a> page for full information on the part.  
 
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_I13453 with GB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_I13453">BBa_I13453</a> page for full information on the part.  
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<br><br>
  
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This is a Level 0 MoClo part with flanking sites G on the 5' side and site B on the 3' side of the part.
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This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part.
 
The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
 
The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
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<br><br>
  
 
Summary of modifications from original part:
 
Summary of modifications from original part:
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<br><br>
  
 
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href="https://parts.igem.org/Part:BBa_K783055">BBa_K783055 </a>.
 
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href="https://parts.igem.org/Part:BBa_K783055">BBa_K783055 </a>.
  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
 
===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
 
 
<partinfo>BBa_K1114032 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1114032 SequenceAndFeatures</partinfo>
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===Source===
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iGEM Distribution Kit
  
  
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<!-- Uncomment this to enable Functional Parameter display
 
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===Functional Parameters===
 
 
<partinfo>BBa_K1114032 parameters</partinfo>
 
<partinfo>BBa_K1114032 parameters</partinfo>
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Latest revision as of 05:59, 25 September 2013

The MoClo format of BBa_I13453 with GB fusion sites.

This is the MoClo formatted part of BBa_I13453 with GB fusion sites. See the BBa_I13453 page for full information on the part.

This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part:

Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 .

Usage and Biology


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 129
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 69
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

iGEM Distribution Kit