Difference between revisions of "Part:BBa K1114005"
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<partinfo>BBa_K1114005 short</partinfo> | <partinfo>BBa_K1114005 short</partinfo> | ||
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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23104 with AB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_J23104">BBa_J23104</a> page for full information on the part. | This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23104 with AB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_J23104">BBa_J23104</a> page for full information on the part. | ||
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This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. | This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. | ||
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. | The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. | ||
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Summary of modifications from original part: | Summary of modifications from original part: | ||
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Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href="https://parts.igem.org/Part:BBa_K783055">BBa_K783055 </a>. | Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href="https://parts.igem.org/Part:BBa_K783055">BBa_K783055 </a>. | ||
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===Source=== | ===Source=== | ||
Revision as of 05:28, 25 September 2013
The MoClo format of BBa_J23104 with AB fusion sites.
This is the MoClo formatted part of BBa_J23104 with AB fusion sites. See the BBa_J23104 page for full information on the part.
This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 .
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Source
iGEM Distribution Kit