Difference between revisions of "Part:BBa K1031742"

 
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109-PaaX-TT
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== '''Construction''' ==
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In order to fine-tune the performance of PaaX biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031742 is composed of three elements, the constitutive ''Pc'' promoter J23109[https://parts.igem.org/Part:BBa_J23109], coding sequence of PaaX and terminator B0015[https://parts.igem.org/Part:BBa_B0015]. ('''Fig 1''')
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/f/fe/Peking2013_part_109-PaaX-TT.png/800px-Peking2013_part_109-PaaX-TT.png", width=600px; />
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'''Fig 1''' Construction of biosensor circuit ''Pa''/J23109-PaaX
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== '''Sequence and Features''' ==
  
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===Usage and Biology===
 
  
 
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===Functional Parameters===
 
===Functional Parameters===
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Revision as of 16:52, 24 September 2013

J23109-PaaX


Construction

In order to fine-tune the performance of PaaX biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031742 is composed of three elements, the constitutive Pc promoter J23109[1], coding sequence of PaaX and terminator B0015[2]. (Fig 1)

Fig 1 Construction of biosensor circuit Pa/J23109-PaaX


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 344
    Illegal NgoMIV site found at 625
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 851
    Illegal SapI.rc site found at 1508