Difference between revisions of "Part:BBa K1073022:Experience"

Revision as of 10:08, 24 September 2013

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Applications of BBa_K1073022

When eforRed is expressed, the colonies on plates as well as the liquid culture become red. The iGEM Team Braunschweig 2013 used this device to differentiate two different strains from one approach.

User Reviews

UNIQaea60769f42c419e-partinfo-00000000-QINU

BBa_K1073022 iGEM Team Braunschweig 2013

The chromoprotein eforRed has a distinct red color. In order to avoid absoption of the chomoproteins during measurement with the photometer, the absorption and emission spectra were measured.

absorption maximum for eforRed: 534 nm

emission maximum for eforRed: 605,4 nm

Accordingly the photometer measurements for growth curves were carried out by 520 nm with respect to the chromoproteins amilGFP (BBa_K1073024) and aeBlue (BBa_K1073020).

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UNIQaea60769f42c419e-partinfo-00000002-QINU

Revision as of 10:07, 24 September 2013 (view source) Kerstin K (Talk \| contribs) (→‎User Reviews) ← Older edit		Revision as of 10:08, 24 September 2013 (view source) Kerstin K (Talk \| contribs) (→‎User Reviews) Newer edit →
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	absorption maximum for eforRed: 534 nm		absorption maximum for eforRed: 534 nm
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	emission maximum for eforRed: 605,4 nm		emission maximum for eforRed: 605,4 nm