Difference between revisions of "Part:BBa K1031916"

Revision as of 09:48, 24 September 2013

J23114-XylS-Terminator

Characterization

In order to fine-tune the performance of XylS biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031912 is composed of three elements, the constitutive Pc promoter J23105[1], coding sequence of XylS and terminator B0015[2]. (Fig 1)

Fig 1 Construction of biosensor circuit Pm/J23105-XylS

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 93
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 815
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 278

Data shown

Induction ratio of XylS biosensor library from ON/OFF test that utilizes different Pc promoters to control the expression of XylS. The dashed box refers to data for Pm/J23114-XylS biosensor circuit (Fig 2)

Fig 2 Horizontal axis stands for different XylS biosensor with Pc promoters of different strength. The expression strength of these constitutive promoters, J23113, J23109, J23114, J23105, J23106 is 21, 106, 256, 623, and 1185, respectively, according to the Partsregistry. Four kinds of aromatics, namely BzO, 2-MxeBzO, 3-MeBzO and 4-MeBzO, shown with different color intensities, were tested following Test Protocol 1 [1]. Vertical axis represents the ON-OFF induction ratio. The dashed box refers to performance for Pm/J23114-XylS biosensor circuit

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1031916 short</partinfo>
--XylS-TT
+<!-- -->
+== '''Characterization''' ==
+In order to fine-tune the performance of XylS biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031912 is composed of three elements, the constitutive ''Pc'' promoter J23105[https://parts.igem.org/Part:BBa_J23105], coding sequence of XylS and terminator B0015[https://parts.igem.org/Part:BBa_B0015]. ('''Fig 1''')
+<html>
+<img src="https://static.igem.org/mediawiki/2013/thumb/3/33/Peking2013_part_114-XylS.png/800px-Peking2013_part_114-XylS.png", width=600px; />
+</html>
+'''Fig 1''' Construction of biosensor circuit ''Pm''/J23105-XylS
+== '''Sequence and Features''' ==
-<!-- Add more about the biology of this part here
-===Usage and Biology===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1031916 SequenceAndFeatures</partinfo>
+== '''Data shown''' ==
+Induction ratio of XylS biosensor library from ON/OFF test that utilizes different Pc promoters to control the expression of XylS. The dashed box refers to data for ''Pm''/J23114-XylS biosensor circuit ('''Fig 2''')
+<html>
+<img src="https://static.igem.org/mediawiki/2013/thumb/8/80/Peking2013_part_Pc_library_114.png/800px-Peking2013_part_Pc_library_114.png", width=600px; />
+</html>
+'''Fig 2'''  Horizontal axis stands for different XylS biosensor with Pc promoters of different strength. The expression strength of these constitutive promoters, J23113, J23109, J23114, J23105, J23106 is 21, 106, 256, 623, and 1185, respectively, according to the Partsregistry. Four kinds of aromatics, namely BzO, 2-MxeBzO, 3-MeBzO and 4-MeBzO, shown with different color intensities, were tested following Test Protocol 1 [1]. Vertical axis represents the ON-OFF induction ratio. The dashed box refers to performance for ''Pm''/J23114-XylS biosensor circuit
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
-<partinfo>BBa_K1031916 parameters</partinfo>
+<partinfo>BBa_K1031912 parameters</partinfo>
 <!-- -->