Difference between revisions of "Part:BBa K1114011:Design"

(Created page with "<partinfo> BBa_K1114011 </partinfo> <partinfo> BBa_K1114011 SequenceAndFeatures </partinfo> <html> This is the <a href=”http://2013.igem.org/Team:BostonU/MoCloChara”>MoClo...")
 
 
Line 1: Line 1:
−
<partinfo> BBa_K1114011 </partinfo>
+
__NOTOC__
−
<partinfo> BBa_K1114011 SequenceAndFeatures </partinfo>
+
<partinfo>BBa_K1114011 short</partinfo>
  
  
 +
<partinfo>BBa_K1114011 SequenceAndFeatures</partinfo>
  
 +
 +
 +
 +
===Design Notes===
 
<html>
 
<html>
−
This is the <a href=”http://2013.igem.org/Team:BostonU/MoCloChara”>MoClo</a> formatted part of BBa_J23108 with AB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_J23108">BBa_J23108</a> page for full information on the part.  
+
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23108 with AB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_J23108">BBa_J23108</a> page for full information on the part.  
−
</html>
+
  
−
This is a MoClo level 0 promoter with a flanking site A on the 5' side and site B on the 3' side of the part.
 
  
−
The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
+
 
 +
This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part.
 +
The fusion site letters refer to 4bp fusion sites: A       = GGAG;       B       = TACT; C       = AATG; D       = AGGT;       E       = GCTT; F       = CGCT; G       = TGCC; H       = ACTA.
 +
 
  
 
Summary of modifications from original part:
 
Summary of modifications from original part:
−
Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
+
 
 +
 
 +
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href="https://parts.igem.org/Part:BBa_K783055">BBa_K783055 </a>.
 +
 
 +
 
 +
</html>
 +
 
 +
 
 +
===Source===
 +
 
 +
 
 +
iGEM Distribution Kit
 +
 
 +
 
 +
===References===

Latest revision as of 18:47, 7 September 2013

The MoClo format of BBa_J23108 with AB fusion sites.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Design Notes

This is the MoClo formatted part of BBa_J23108 with AB fusion sites. See the BBa_J23108 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 .


Source

iGEM Distribution Kit


References