Difference between revisions of "Part:BBa K1114008:Design"

(Created page with "<html> This is the <a href=”http://2013.igem.org/Team:BostonU/MoCloChara”>MoClo</a> formatted part of BBa_J23105 with EB fusion sites. See the <a href="https://parts.igem.org/...")
 
 
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<partinfo>BBa_K1114008 short</partinfo>
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<partinfo>BBa_K1114008 SequenceAndFeatures</partinfo>
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===Design Notes===
 
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This is the <a href=”http://2013.igem.org/Team:BostonU/MoCloChara”>MoClo</a> formatted part of BBa_J23105 with EB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_J23105">BBa_J23105</a> page for full information on the part.  
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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23105 with EB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_J23105">BBa_J23105</a> page for full information on the part.  
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This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part.
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The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
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Summary of modifications from original part:
 
Summary of modifications from original part:
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Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
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The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
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Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href="https://parts.igem.org/Part:BBa_K783056">BBa_K783056 </a>.
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</html>
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===Source===
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iGEM Distribution Kit
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===References===

Latest revision as of 18:42, 7 September 2013

The MoClo format of BBa_J23105 with EB fusion sites.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Design Notes

This is the MoClo formatted part of BBa_J23105 with EB fusion sites. See the BBa_J23105 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783056 .


Source

iGEM Distribution Kit


References