Difference between revisions of "Part:BBa K1114005"

 
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__NOTOC__
 
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<partinfo>BBa_K1114005 short</partinfo>
 
<partinfo>BBa_K1114005 short</partinfo>
  
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<partinfo>BBa_K1114005 SequenceAndFeatures</partinfo>
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===Design Notes===
 
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<html>
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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_R0079 with EB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_R0079">BBa_R0079</a> page for full information on the part.  
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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23104 with AB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_J23104">BBa_J23104</a> page for full information on the part.  
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This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part.
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The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
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Summary of modifications from original part:
 
Summary of modifications from original part:
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Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
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Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href="https://parts.igem.org/Part:BBa_K783055">BBa_K783055 </a>.
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===Source===
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
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iGEM Distribution Kit
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1114005 SequenceAndFeatures</partinfo>
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===References===
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===Functional Parameters===
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<partinfo>BBa_K1114005 parameters</partinfo>
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Revision as of 18:38, 7 September 2013

The MoClo format of BBa_J23104 with AB fusion sites.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Design Notes

This is the MoClo formatted part of BBa_J23104 with AB fusion sites. See the BBa_J23104 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 .


Source

iGEM Distribution Kit


References