Difference between revisions of "Part:BBa K1114012:Design"
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===Design Notes=== | ===Design Notes=== | ||
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===Design Notes=== | ===Design Notes=== | ||
Latest revision as of 18:10, 7 September 2013
The MoClo format of BBa_J23109 with AB fusion sites.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The MoClo format of BBa_J23109 with AB fusion sites.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is the MoClo formatted part of BBa_J23109 with FB fusion sites. See the BBa_J23109 page for full information on the part.
This is a Level 0 MoClo part with flanking sites F on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
Source
iGEM Distribution Kit
References
Source
iGEM Distribution Kit