Difference between revisions of "Part:BBa K801060:Design"

(→‎References)
(→‎References)
Line 95: Line 95:
 
*[http://www.ncbi.nlm.nih.gov/nuccore/21435702 '''GenBank''': source of the sequence]
 
*[http://www.ncbi.nlm.nih.gov/nuccore/21435702 '''GenBank''': source of the sequence]
 
<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
 
<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
−
<!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]-->
+
*[http://www.uniprot.org/uniprot/Q8L5K3 '''Uniprot''': (R)-limonene synthase 1]
 
<!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
 
<!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
 
<!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
 
<!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->

Revision as of 11:44, 15 October 2012

(+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1683
    Illegal SapI.rc site found at 107


Keywords: limonene synthase, limonene


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

  • Biobrick was sequenced

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:

  • BioBrick encodes (+)-limonene synthase
  • UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]

Enzymatic activity:

  • E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]

Cytotoxicity:

  • see toxicity assay

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

  • BioBrick: BBa_I742111
  • Forward Primer: TUM12-LIMS1-fw-cons: 5'- GGATCCTCTAGAGTACACAATGCGTCGTTCAGCAAAC - 3'
  • Reverse Primer: TUM12-LIMS1-rv: 5'- GGATCCACCGGTGCCTTTGGTGCCAGGAGA - 3'

Organism:

  • Gene sequence derived from Citrus limon

References

Literature references:

  • [http://www.ncbi.nlm.nih.gov/pubmed/12084056Pubmed: Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A., 2002: Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71.]

Database references:

  • [http://www.ncbi.nlm.nih.gov/nuccore/21435702 GenBank: source of the sequence]
  • [http://www.uniprot.org/uniprot/Q8L5K3 Uniprot: (R)-limonene synthase 1]