Difference between revisions of "Part:BBa K801060:Design"
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Revision as of 18:35, 3 October 2012
(+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1683
Illegal SapI.rc site found at 107
Keywords:
limonene synthase, limonene
Abbreviations:
Design Notes
Related BioBrick:
- BBa_K801061: (+)-Limonene synthase 1 coding region
- BBa_K801062: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801063: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801064: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801065: (+)-limonene synthase 1 with Strep-Tag
- BBa_K801066: (+)-limonene synthase 1 with Yeast Consensus Sequence for improved expression
Quality control measures:
- Biobrick was sequenced
Backbone:
- Backbone name: pSB1C3
- Resistance: Cp
- Copynumber: high
Protein coding:
- BioBrick encodes (+)-limonene synthase
- UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]
Enzymatic activity:
- E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]
Cytotoxicity:
Safety notes:
Intellectual property:
Corresponding part author/authors:
Source
Source:
- BioBrick: BBa_I742111
- Forward Primer: TUM12-LIMS1-fw-cons: 5'- GGATCCTCTAGAGTACACAATGCGTCGTTCAGCAAAC - 3'
- Reverse Primer: TUM12-LIMS1-rv: 5'- GGATCCACCGGTGCCTTTGGTGCCAGGAGA - 3'
Organism:
- Gene sequence derived from Citrus limon
References
Literature references:
- http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002 Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71.
Database references:
- [http://www.ncbi.nlm.nih.gov/nuccore/21435702 GenBank: source of the sequence]