Difference between revisions of "Part:BBa K801065:Design"

(→‎Source)
(→‎Design Notes)
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'''Backbone:'''<br>
 
'''Backbone:'''<br>
−
*Backbone name: pSB1C3'
+
* Backbone name: pSB1C3
−
<!--*Resistance: Amp/Cp/Kan/Tet-->
+
* Resistance: Cp
−
<!--*Copynumber: low/medium/high-->
+
* Copynumber: high
  
 
'''Protein coding:'''<br>
 
'''Protein coding:'''<br>
−
<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
+
* BioBrick encodes (+)-limonene synthase
−
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
+
* UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]
−
<!--*The protein encoded is posttranslationally modified by ???.-->
+
<!--none/not known/cytotoxic for ''organism name''-->
−
<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
+
  
 
'''Enzymatic activity:'''
 
'''Enzymatic activity:'''
−
EC-number 4.2.3.20
+
* E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]
−
 
+
 
'''Cytotoxicity:'''<br>
 
'''Cytotoxicity:'''<br>
 
<!--none/not known/cytotoxic for ''organism name''-->
 
<!--none/not known/cytotoxic for ''organism name''-->

Revision as of 18:30, 3 October 2012

(+)-limonene synthase 1 with Strep-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1676
    Illegal SapI.rc site found at 100


C-Terminal Strep-Tag added


See BBa_K801061


Keywords: limonen, strep-tag


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:

  • BioBrick encodes (+)-limonene synthase
  • UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]

Enzymatic activity:

  • E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

  • BioBrick: BBa_I742111


Organism:

  • Gene sequence derived from Citrus limon

References

Literature references:

  • http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002 Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71.

Database references: