Difference between revisions of "Part:BBa K801062:Design"

(→‎Design Notes)
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'''Backbone:'''<br>
 
'''Backbone:'''<br>
−
<!--*Backbone name: pSB1C3'/?backbone_name?-->
+
*Backbone name: pSB1C3'
 
<!--*Resistance: Amp/Cp/Kan/Tet-->
 
<!--*Resistance: Amp/Cp/Kan/Tet-->
 
<!--*Copynumber: low/medium/high-->
 
<!--*Copynumber: low/medium/high-->
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'''Enzymatic activity:'''
 
'''Enzymatic activity:'''
−
<!--none/EC-number ?.?.?.?-->
+
*EC-number 4.2.3.20
  
 
'''Cytotoxicity:'''<br>
 
'''Cytotoxicity:'''<br>
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'''Organism:'''<br>
 
'''Organism:'''<br>
−
<!--*Genesequence derived from ''?organism_name?''-->
+
<!--*Genesequence derived from '' Citrus limon''-->
 
<!--*Codonoptimized for ''?organism_name?''-->
 
<!--*Codonoptimized for ''?organism_name?''-->
 
<!--*Designed for the following Chassis: ''?organism-name?''-->
 
<!--*Designed for the following Chassis: ''?organism-name?''-->

Revision as of 15:27, 3 October 2012

(+)-limonene synthase 1 expression cassette for yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2127
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 211
    Illegal SapI.rc site found at 561


This is a composite part consisting of the yeast TEF1 promoter (BBa_K319003), the Citrus limon (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast CYC1 terminator (BBa_K122003).


Keywords:


Abbreviations:

Design Notes

Related BioBrick:


Quality control measures:

Backbone:

  • Backbone name: pSB1C3'

Protein coding:

Enzymatic activity:

  • EC-number 4.2.3.20

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: