Difference between revisions of "Part:BBa K801060:Design"
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'''Backbone:'''<br> | '''Backbone:'''<br> | ||
| − | + | * Backbone name: pSB1C3 | |
| − | + | * Resistance: Cp | |
| − | + | * Copynumber: high | |
'''Protein coding:'''<br> | '''Protein coding:'''<br> | ||
Revision as of 12:56, 3 October 2012
(+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1683
Illegal SapI.rc site found at 107
Keywords:
limonene synthase, limonene
Abbreviations:
Design Notes
Related BioBrick:
- BBa_K801061: (+)-Limonene synthase 1 coding region
- BBa_K801062: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801063: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801064: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801065: (+)-limonene synthase 1 with Strep-Tag
- BBa_K801066: (+)-limonene synthase 1 with Yeast Consensus Sequence for improved expression
Quality control measures:
Backbone:
- Backbone name: pSB1C3
- Resistance: Cp
- Copynumber: high
Protein coding:
Enzymatic activity:
Cytotoxicity:
This Biobrick leads to the production of limonene. We investigated the toxicity of limonene and found out, that the growth of yeast strains was inhibited at concentrations of 1 mM. Limonene with a concentration of 100 mM even showed a lethal effect. Lower concentrations of limonene (range of micro molar) did not show any growth inhibition or effect.
Safety notes:
Intellectual property:
Corresponding part author/authors:
Source
Source:
Organism:
References
Literature references:
Database references:
- [http://www.ncbi.nlm.nih.gov/nuccore/21435702 GenBank: source of the sequence]