Difference between revisions of "Part:BBa K801060:Design"
(→‎Design Notes)
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'''Related BioBrick:'''
 
'''Related BioBrick:'''
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* [https://parts.igem.org/Part:BBa_K801061 BBa_K801061: (+)-Limonene synthase 1 coding region]
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* [https://parts.igem.org/Part:BBa_K801062 BBa_K801062: (+)-limonene synthase 1 expression cassette for yeast]
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* [https://parts.igem.org/Part:BBa_K801063 BBa_K801063: (+)-limonene synthase 1 expression cassette for yeast]
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* [https://parts.igem.org/Part:BBa_K801064 BBa_K801064: (+)-limonene synthase 1 expression cassette for yeast]
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* [https://parts.igem.org/Part:BBa_K801065 BBa_K801065: (+)-limonene synthase 1 with Strep-Tag]
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* [https://parts.igem.org/Part:BBa_K801066 BBa_K801066: (+)-limonene synthase 1 with Yeast Consensus Sequence for improved expression]
 
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<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
 
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]

Revision as of 12:45, 3 October 2012

(+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1683
    Illegal SapI.rc site found at 107


Keywords: limonene synthase, limonene


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:
This Biobrick leads to the production of limonene. We investigated the toxicity of limonene and found out, that the growth of yeast strains was inhibited at concentrations of 1 mM. Limonene with a concentration of 100 mM even showed a lethal effect. Lower concentrations of limonene (range of micro molar) did not show any growth inhibition or effect.

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references:

  • [http://www.ncbi.nlm.nih.gov/nuccore/21435702 GenBank: source of the sequence]