Difference between revisions of "Part:BBa K844015"
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<partinfo>BBa_K844015 short</partinfo> | <partinfo>BBa_K844015 short</partinfo> | ||
| − | This part is a spider silk 1x "F" subunit with GFP fused to its C-terminus. This generator is lactose/ITPG inducible. | + | This part is a spider silk 1x "F" subunit ([https://parts.igem.org/Part:BBa_K844007 BBa_K844007]) with GFP ([https://parts.igem.org/Part:BBa_K208000 BBa_K208000]) fused to its C-terminus. This generator is lactose/ITPG inducible. |
IMPORTANT NOTE: This part uses [https://parts.igem.org/Assembly_standard_23 Assembly Standard #23 (Silver Fusion)] scar sites, which the sequence data below will not reflect (it shows [https://parts.igem.org/Assembly_standard_10 Assembly Standard #10] scars). | IMPORTANT NOTE: This part uses [https://parts.igem.org/Assembly_standard_23 Assembly Standard #23 (Silver Fusion)] scar sites, which the sequence data below will not reflect (it shows [https://parts.igem.org/Assembly_standard_10 Assembly Standard #10] scars). | ||
Revision as of 04:32, 3 October 2012
lac/IPTG inducible Spider Silk 1x "F" Subunit fused to GFP
This part is a spider silk 1x "F" subunit (BBa_K844007) with GFP (BBa_K208000) fused to its C-terminus. This generator is lactose/ITPG inducible.
IMPORTANT NOTE: This part uses Assembly Standard #23 (Silver Fusion) scar sites, which the sequence data below will not reflect (it shows Assembly Standard #10 scars).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 443
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 962
Illegal XhoI site found at 863 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]