Difference between revisions of "Part:BBa K844016"

Revision as of 03:58, 3 October 2012

Spider Silk Generator - 4x "B" Silk Construct with His Tag

Protein generator part for Spider Silk. Protein is a 4x fusion of spider silk "B" subunits, which were codon optimized to use a reduced set of tRNA codons (1 to 2 codons per amino acid). Contains a lactose/IPTG inducible , RBS, and a 10x-His tag for purification of protein.

IMPORTANT NOTE: This part uses Assembly Standard #23 (Silver Fusion) scar sites, which the sequence data below will not reflect (it shows Assembly Standard #10 scars).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K844016 short</partinfo>
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 Protein generator part for Spider Silk. Protein is a 4x fusion of spider silk "B" subunits, which were codon optimized to use a reduced set of tRNA codons (1 to 2 codons per amino acid). Contains a lactose/IPTG inducible , RBS, and a 10x-His tag for purification of protein.
-IMPORTANT NOTE: This part uses Assembly Standard #23 (Silver Fusion) scar sites, which the sequence data below will not reflect (it shows Assembly Standard #10 scars).
+IMPORTANT NOTE: This part uses [https://parts.igem.org/Assembly_standard_23 Assembly Standard #23 (Silver Fusion)] scar sites, which the sequence data below will not reflect (it shows [https://parts.igem.org/Assembly_standard_10 Assembly Standard #10] scars).
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