Difference between revisions of "Part:BBa I742111:Experience"

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We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as <partinfo>K562015</partinfo>). This will allow better monitoring of production of the protein, and it was detectable using an anti-HA monoclonal antibody whenexpressed in <i>E. coli</i>. We did not get the chance to test limonene production in a host cell producing this part.
 
We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as <partinfo>K562015</partinfo>). This will allow better monitoring of production of the protein, and it was detectable using an anti-HA monoclonal antibody whenexpressed in <i>E. coli</i>. We did not get the chance to test limonene production in a host cell producing this part.
 
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<partinfo>BBa_K190019 AddReview 2</partinfo>
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<I>iGEM Wisconsin-Madison 2012</I>
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The sequence of this part matched the sequence listed. However, when we used this gene for the production of limonene, we were not able to produce any in <i>E. coli</i>. We thought a problem may be rare codons in the sequence so we codon optimized the sequence, and sent it in with our parts this year (Bba_K762100). Our GC/MS data did show a similar peak near the expected limonene ion, but unfortunately the same peak came up for our empty promoter.
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Revision as of 16:46, 30 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I742111

A composite LIMS1 part with a Lac promoter has been characterized. For details, please visit this page.

Team TU Munich 2012 used this part as PCR-template to obtain the coding sequence of (+)-Limonene Synthase. See the page of our BioBrick BBa_K801060.

User Reviews

UNIQd14e486135e30a12-partinfo-00000002-QINU


•••

iGEM Dundee 2011

We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as BBa_K562015). This will allow better monitoring of production of the protein, and it was detectable using an anti-HA monoclonal antibody whenexpressed in E. coli. We did not get the chance to test limonene production in a host cell producing this part.

••

iGEM Wisconsin-Madison 2012

The sequence of this part matched the sequence listed. However, when we used this gene for the production of limonene, we were not able to produce any in E. coli. We thought a problem may be rare codons in the sequence so we codon optimized the sequence, and sent it in with our parts this year (Bba_K762100). Our GC/MS data did show a similar peak near the expected limonene ion, but unfortunately the same peak came up for our empty promoter.

UNIQd14e486135e30a12-partinfo-00000006-QINU